Synergistic augmentative effect of interleukin‐10 and CD27/CD70 interactions on B‐cell immunoglobulin synthesis

Publisher Website
Google Scholar
Abstract
Interleukin‐10 (IL‐10) is a potent cytokine that regulates immunoglobulin synthesis by B cells. CD27/CD70 interactions by direct cell‐to‐cell contact are also needed to produce substantial amounts of immunoglobulin. We have investigated the effects of IL‐10 and CD27/CD70 interactions on the immunoglobulin synthesis. In the presence of IL‐10 stimulation, the production of IgG, IgM and IgA was increased synergistically by the addition of CD27 ligand (CD70)‐transfectants in a dose‐dependent manner, which was completely blocked by anti‐CD70 monoclonal antibody. In contrast, CD70‐transfectants additively enhanced the immunoglobulin production in the presence of IL‐2, IL‐4, or IL‐6. The synergistic enhancement of the immunoglobulin production by IL‐10 and CD70‐transfectants was remarkable in highly purified CD27+ B cells, but there was no immunoglobulin production in CD27− B cells. Furthermore, by the addition of CD70‐transfectants, the synthesis of IgG1, IgG2, IgG3 and IgG4 was also enhanced in the presence of IL‐10. On the other hand, IL‐10 diminished CD27 expression in B cells. B‐cell proliferation was augmented by CD70‐transfectants with IL‐10 or IL‐10 plus IL‐2. The addition of IL‐2 further augmented the immunoglobulin production which was synergistically enhanced by IL‐10 and CD27 triggering. Taken together, the co‐operative response to IL‐10 and CD27/CD70 interactions regulates B‐cell immunoglobulin production.