DNA bis‐intercalation: Application of theory to the binding of echinomycin to DNA
- 1 February 1980
- journal article
- research article
- Published by Wiley in Biopolymers
- Vol. 19 (2) , 431-443
- https://doi.org/10.1002/bip.1980.360190216
Abstract
The statistical mechanical model for the binding of bifunctional intercalating ligands to duplex DNA described in the preceding paper is applied to the example of echinomycin–DNA interactions. This is the only system for which binding curves have been obtained under conditions leading to binding by both bis‐intercalation and mono‐intercalation simultaneously. Binding parameters and Scatchard plots are calculated for a variety of conditions. A detailed comparison of these calculations with the results from the previous analysis of the same binding data in terms of the McGhee‐Von Hippel theory, assuming only one mode of binding, is presented. The results of our calculations are consistent with the model of bis‐intercalation requiring the two bound chromophores of a bifunctional ligand to be separated by two base pairs. It is not necessary to assume violation of the nearest‐neighbor exclusion principle, as occurred in the original analysis.This publication has 23 references indexed in Scilit:
- DNA bis‐intercalation: Theoretical calculation of binding curvesBiopolymers, 1980
- DNA bifunctional intercalators. 2. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer. Appendix: Numerical solution of McGhee and von Hippel equations for competing ligandsBiochemistry, 1978
- Potential antitumor agents. 28. Deoxyribonucleic acid polyintercalating agentsJournal of Medicinal Chemistry, 1978
- Molecular recognition of DNA by small molecules. Synthesis of bis(methidium)spermine, a DNA polyintercalating moleculeJournal of the American Chemical Society, 1978
- Synthesis, DNA-binding and biological activity of a double intercalating analog of ethidium bromideNucleic Acids Research, 1978
- The binding of echinomycin to deoxyribonucleic acidBiochemical Journal, 1976
- Diacridines: Bifunctional intercalators I. Chemistry, physical chemistry and growth inhibitory propertiesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- DNA polyintercalating drugs: DNA binding of diacridine derivatives.Proceedings of the National Academy of Sciences, 1975
- Structure revision of the antibiotic echinomycinJournal of the American Chemical Society, 1975
- Echinomycin: a bifunctional intercalating antibioticNature, 1974