THE RESPIRATION OF ISOLATED RAT-HEPATIC CELLS IN SUSPENSION

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Abstract
Rat-hepatic cells in suspension have been shown to have an endogenous respiration of 5.6 [plus and minus] 0.17 when suspended in 0.1 M-sucrose and 0.02 M-tris-HCl buffer. The respiration in 0.25 M-sucrose and 0.02 M-tris-HC1 buffer is 30-40% less. Potassium chloride (0.05 M) is slightly inhibitory and CaCl2 (0.0025 M) highly inhibitory to endogenous respiration of the hepatic cells in suspension. The cells do not respire in Krebs-Ringer phosphate buffer. The respiration of the hepatic cells in suspension is stimulated by pyruvate, citrate, isocitrate, oxoglutarate, succinate, fumarate, malate and glutamate; there is no significant stimulation (or inhibition) by glucose, fructose, acetate and butyrate. In almost all the cases where stimulation was observed, it was found that the higher the endogenous respiration the lower is the stimulation.