Characterization of Pathogenic Escherichia coli Isolated from Humans in Austria: Phenotypes, Toxin Gene Types and Epidemiology

Publisher Website
Google Scholar
Abstract
Summary: One hundred and ten clinical Escherichia coli isolates of serovar O157 (n = 102) and O26 (n = 8) were characterized for the presence of putative virulence genes by PCR. All but one of these isolates contained the eae gene. The EHEC‐hly gene could be detected in all E. coli O157 and in 50% of E. coli O26 isolates. Forty‐five (40.9%) of the 110 E. coli were positive for both stx1 and stx2 genes, 2 (1.8%) isolates were positive for stx1 and 57 isolates (51.8%) were positive for stx2 only. Among the 102 stx2 positive isolates, 14 (13.7%) E. coli O157 contained also the stx2c variant gene. No other stx2 variant was identified. Six clinical isolates (five E. coli O157:H7 and one E. coli O26) did not contain stx genes. Ten non‐pathogenic E. coli isolates which were amplified as controls didn't contain any stx and eae gene but two of the ten strains contained the EHEC‐hly gene. By their growth on chromogenic media, all but two of 50 E. coli O157 could be differentiated from eight E. coli O26 and 10 non‐pathogenic E. coli. Sixty‐one of the O157:H7 isolates were further subjected to pulsed‐field gel electrophoresis (PFGE) which identified 49 distinguishable patterns. In five cases where contact infection among family members was suspected, indistinguishable PFGE patterns confirmed the epidemiological relatedness of the isolates. Moreover, two PFGE clusters were identified which comprised five and three strains, respectively. These findings indicate the occurrence of both family and diffuse outbreaks of E. coli O157 infections in Austria during recent years and demonstrate the need for molecular subtyping of these pathogens.