A P2X purinoceptor cDNA conferring a novel pharmacological profile
- 9 March 2000
- journal article
- research article
- Published by Wiley
- Vol. 375 (1-2) , 129-133
- https://doi.org/10.1016/0014-5793(95)01203-q
Abstract
We have cloned P2X4, a member of the P2‐purinoceptor family, which has a new pharmacological profile. Rat P2X4 is distantly related to P2X1, P2X2 and P2X3 and is expressed in brain, spinal cord, lung, thymus, bladder, adrenal, testis and vas deferens. This ligand gated ion channel is activated by ATP and analogs with the potency order of ATP > ATPγS > 2‐methylthio ATP > ADP ≈ αβ‐methylene ATP. However, none of the currently used P2X purinoceptor antagonists suramin, reactive blue 2 and PPADS blocked ATP evoked currents; in contrast their application resulted in potentiation of the agonist response. Due to lack of any known antagonist for P2X4 it is unlikely that native P2X4 has previously been recognized as a P2X purinoceptor.Keywords
This publication has 20 references indexed in Scilit:
- Facilitation of Hippocampal Potentials by SuraminJournal of Neurochemistry, 2002
- Is there a basis for distinguishing two types of P2-purinoceptor?Published by Elsevier ,2002
- P2x receptors bring new structure to ligand-gated ion channelsPublished by Elsevier ,1999
- A P2X purinoceptor expressed by a subset of sensory neuronsNature, 1995
- Distribution of [3H]α,β-methylene ATP binding sites in rat brain and spinal cordNeuroReport, 1994
- G protein-coupled receptors for ATP and other nucleotides: a new receptor familyTrends in Pharmacological Sciences, 1994
- Signalling via ATP in the nervous systemTrends in Neurosciences, 1994
- Purinoceptors: Are there families of P2X and P2Y purinoceptors?Pharmacology & Therapeutics, 1994
- ATP ‐ a fast neurotransmitterFEBS Letters, 1993
- Physiological and pathological roles of purines: An updateDrug Development Research, 1993