SHEDDING AND REAPPEARANCE OF FC, C3 AND SRBC RECEPTORS ON PERIPHERAL LYMPHOCYTES FROM NORMAL DONORS AND CHRONIC LYMPHATIC-LEUKEMIA (CLL) PATIENTS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 34  (2) , 315-321
Abstract
The shedding and resynthesis of Fc, C3 [3rd component of complement] receptors and receptors binding SRBC [sheep red blood cells] was studied in normal peripheral lymphocytes, in lymphocytes from CLL patients and in a [human Burkitt''s lymphoma] Raji cell line during 4 h incubation at 37.degree. C. During the 1st part of the incubation, an increased release of receptors was observed. The shed, functionally active, receptors were detectable in the incubating medium on the basis of their ability to inhibit rosette formation specifically. After 4 h incubation the original ratios of the different rosette forming cells were observed, showing that the cells were capable of resynthesizing their shed receptors. This later phenomenon may be the result of an active metabolic process. The shedding was not detectable on Raji cells. Different patterns of receptor shedding and resynthesis were demonstrated by the lymphocytes of CLL patients. The correlations between the reappearance of the receptors on the cells, and the metabolism of the lymphocytes, is discussed.