In vitro translation studies of the cytoplasmic nonpolysomal particles containing messenger RNA
Open Access
- 25 April 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (8) , 1793-1804
- https://doi.org/10.1093/nar/8.8.1793
Abstract
We analyzed the translational capacity of different kinds of free cytoplasmic messenger ribonucleoprotein complexes (free mRNP) in a Hela cell cell free system. Native free mRNP are not translated although free mRNP washed with 0.5 M KCl can direct polypeptide synthesis. Furthermore, the 0.5 M KCl wash possesses a factor which inhibits the translation of 0.5 M KCl washed free mRNP as well as globin mRNA naked mRNA from plasmocytoma, or Hela cells. We also demonstrated that native free mRNP are able to form a complex with ribosomal subunits in the presence of initiation factors. This indicates that inhibition of translation by the 0.5 M KCl wash occurs either at some point after initiation complex formation or at the elongation step.Keywords
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