Development of a Normal Human Immunoglobulin G Standard Curve for Enzyme‐Linked Immunosorbent Assay: Use for Comparison of Antigen Efficacy

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Abstract
Internal standard reference curves are used in enzyme‐linked immunosorbent assay (ELISA) plates to control for inter‐ and intra‐assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5°C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development.