NUCLEIC ACID CONTENT OF HeLa S3 CELLS DURING THE CELL CYCLE: VARIATIONS BETWEEN CYCLES

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Abstract
The process of continuous resynchronization with excess thymidine provides sufficient cell material for accurate chemical determination of DNA and RNA in HeLa [human cervical carcinoma cells] S3 cells at hourly intervals during the cell cycle. Total DNA is constant during the non-S phase portion of the cell cycle but varies widely among cycles of synchronous growth. Total cellular RNA content increases linearly in the G1 phase and accelerates to a higher linear rate of accumulation, which remains constant during most of the S and G2 phases. The ratios of early and late cycle rates of RNA accumulation are not constant among cycles.