Use of CD137 to study the full repertoire of CD8+ T cells without the need to know epitope specificities
Open Access
- 22 October 2008
- journal article
- review article
- Published by Wiley in Cytometry Part A
- Vol. 73A (11) , 1043-1049
- https://doi.org/10.1002/cyto.a.20594
Abstract
CD137 (4‐1BB) is a member of the TNFR‐family with costimulatory function, triggering prosurvival signals in activated T‐cells. Upregulation of CD137 upon stimulation allows identifying and isolating live, human antigen‐specific CD8+ T‐cells of all phenotypes, and therefore provides a comprehensive detection method. Furthermore responses against antigen mixtures can be easily detected, enabling antigen discovery in a stepwise deconvoluting approach. In this article, we will discuss various aspects of this methodology, including potential pitfalls as well as a variety of applications, as illustrated by examples from our laboratory. © 2008 International Society for Advancement of CytometryKeywords
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