Flow field-flow fractionation-inductively coupled plasma mass spectrometry and metal speciation in proteins: A feasibility study
- 1 January 1999
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of Analytical Atomic Spectrometry
- Vol. 14 (9) , 1527-1531
- https://doi.org/10.1039/a901804b
Abstract
The feasibility of trace metal speciation in proteins directly using flow field-flow fractionation-inductively coupled plasma mass spectrometry (FlFFF-ICP-MS) was investigated. The effect on the separation of the FlFFF system cross flow rate was demonstrated for a carbonic anhydrase and alcohol dehydrogenase mixture. Preliminary studies with FlFFF-ICP-MS were applied to several protein standards including metallothionein, carbonic anhydrase, ceruloplasmin, alcohol dehydrogenase, and thyroglobulin. Cadmium was detected in metallothionein; copper and zinc in carbonic anhydrase, alcohol dehydrogenase, and ceruloplasmin, and iodide in thyroglobulin.Keywords
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