Atrial natriuretic factor receptors and stimulation of cyclic GMP formation in normal and malignant osteoblasts
- 2 November 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 208 (2) , 263-268
- https://doi.org/10.1016/0014-5793(86)81029-8
Abstract
Synthetic rat atrial natriuretic factor (Ile‐ANF‐26) stimulated cyclic GMP formation by up to several hundred‐fold in osteoblast‐rich cultures from newborn rat calvaria and in clonal osteogenic sarcoma cells (UMR 106‐01) which are phenotypically osteoblast. ANF had no effect on the cyclic AMP response to parathyroid hormone in the same cells. Specific, high‐affinity binding sites for ANF were identified in both cell types, with Kd and receptor numbers in normal osteoblasts of 1.2 ± 0.1 × 10−10 M and 42 ± 4 × 103 per cell, and in UMR 106‐01 cells of 1.4 ± 0.1 × 10−10M and 22 ± 4 × 103per cell.Keywords
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