HSP70‐related 65 kDa protein of beet yellows closterovirus is a microtubule‐binding protein
- 8 June 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 304 (1) , 12-14
- https://doi.org/10.1016/0014-5793(92)80578-5
Abstract
Beet yellows virus (BYV) genome encodes a 65 kDa protein homologous to the HSP70 family of cellular heat‐shock proteins (Agranovsky, A.A., Boyko, V.P., Karasev, A.V., Koonin, E.V. and Dolja, V.V. (1991) J. Mol. Biol. 217, 603–610). The respective gene was cloned and expressed in vitro yielding a product of the expected size (p65). This product was found to bind to the purified microtubules with a binding constant of 4 × 10−7 M. The binding of p65 was stimulated if ATP presented in the translation mixture was hydrolyzed by apyrase. Removal of the short C‐terminal domains of α‐ and β‐tubulin by subtilisin digestion abolished the binding, demonstrating its specificity. The possible role of p65 association with microtubules in the movement of virus within and/or between plant cells is proposed.Keywords
This publication has 15 references indexed in Scilit:
- Diverse Groups of Plant RNA and DNA Viruses Share Related Movement Proteins that may Possess Chaperone-like ActivityJournal of General Virology, 1991
- MAP2‐mediated binding of chromaffin granules to microtubulesFEBS Letters, 1991
- Putative 65 kDa protein of beet yellows closterovirus is a homologue of HSP70 heat shock proteinsJournal of Molecular Biology, 1991
- Nucleotide Sequence of the 3'-terminal Half of Beet Yellows Closterovirus RNA genome: Unique Arrangement of Eight Virus GenesJournal of General Virology, 1991
- Expression of RNA transcripts of potato virus X full-length and subgenomic cDNAsBiochimie, 1990
- Polypeptide chain binding proteins: Catalysts of protein folding and related processes in cellsCell, 1989
- Movement Protein of Tobacco Mosaic Virus Modifies Plasmodesmatal Size Exclusion LimitScience, 1989
- The Movement of Viruses in PlantsAnnual Review of Phytopathology, 1989
- A 70 kDa microtubule‐associated protein in NIL8 cells comigrates with the 70 kDa heat shock proteinBiology of the Cell, 1988
- Selective dendritic transport of RNA in hippocampal neurons in cultureNature, 1987