Mutations atpipXSuppress Lethality of PII-Deficient Mutants ofSynechococcus elongatusPCC 7942
- 1 August 2009
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 191 (15) , 4863-4869
- https://doi.org/10.1128/jb.00557-09
Abstract
The P(II) proteins are found in all three domains of life as key integrators of signals reflecting the balance of nitrogen and carbon. Genetic inactivation of P(II) proteins is typically associated with severe growth defects or death. However, the molecular basis of these defects depends on the specific functions of the proteins with which P(II) proteins interact to regulate nitrogen metabolism in different organisms. In Synechococcus elongatus PCC 7942, where P(II) forms complexes with the NtcA coactivator PipX, attempts to engineer P(II)-deficient strains failed in a wild-type background but were successful in pipX null mutants. Consistent with the idea that P(II) is essential to counteract the activity of PipX, four different spontaneous mutations in the pipX gene were found in cultures in which glnB had been genetically inactivated.Keywords
This publication has 54 references indexed in Scilit:
- Expression and Mutational Analysis of the glnB Genomic Region in the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120Journal of Bacteriology, 2009
- The crystal structure of the complex of P II and acetylglutamate kinase reveals how P II controls the storage of nitrogen as arginineProceedings of the National Academy of Sciences, 2007
- Interaction of the Membrane-bound GlnK-AmtB Complex with the Master Regulator of Nitrogen Metabolism TnrA in Bacillus subtilisJournal of Biological Chemistry, 2006
- Ammonium assimilation in cyanobacteriaPhotosynthesis Research, 2005
- Complex Formation and Catalytic Activation by the PII Signaling Protein of N-Acetyl-l-glutamate Kinase from Synechococcus elongatus Strain PCC 7942Journal of Biological Chemistry, 2004
- Cell‐type specific modification of PII is involved in the regulation of nitrogen metabolism in the cyanobacterium Anabaena PCC 7120FEBS Letters, 2004
- Interactions between the Nitrogen Signal Transduction Protein PII and N -Acetyl Glutamate Kinase in Organisms That Perform Oxygenic PhotosynthesisJournal of Bacteriology, 2004
- Transcriptional effects of the signal transduction protein PII (glnB gene product) on NtcA‐dependent genes in Synechococcus sp. PCC 7942FEBS Letters, 2003
- 2‐Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoterFEBS Letters, 2002
- Characterization of the glnB gene product of Nostoc punctiforme strain ATCC 29133: glnB or the PII protein may be essentialMicrobiology, 1998