Female Supella longipalpa exhibit a pattern of calling behavior in which the wings are raised and the genitalia are rhythmically opened and closed. Of these calling females, 79% mated when paired with males, whereas only 8% of non calling females mated. Eight of nine mated females ceased to call immediately after mating and did not call through at least two oviposition cycles. Unmated females exhibited cycles of calling and production of infertile eggs. The mean inter-ovipositional period in unmated females was 7.9 days, and it was 3.6 days in mated females. Males began responding to females 8 or 9 days after the females' imaginal molt. Calling and the onset of sexual receptivity were initiated 11 to 15 days after the imaginal molt.