Abstract
The axial gradients in many plants and animals can be very clearly and beautifully demonstrated as color gradients by the use of low concentrations of K2Mn2O8, the color being due to the reduction of the permanganate to MnO2 by protoplasm. Axial gradients have been demonstrated by this method in all axiate organisms thus far examined including several algæ, a diatom pseudothallus, four species of protozoa, various hydroids, hydromedusae and their developmental stages, a siphonophore, actinians, developmental stages of polyclads and echinoderms, axiate appendages of polychetes, and ascidian tadpoles.

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