Enzymatic synthesis of a new type of penicillin

Abstract
A new antibacterial penicillin, containing a 2-α-methoxy group, has been obtained by enzymatic synthesis from the tripeptide δ-(L-α-aminoadipoyl)-L-cysteinyl-D-(O-methyl-allothreonine) with isopenicillin N synthetase derived from Cephalosporium acremonium CO 728[δ-(L-α-aminoadipoyl)= 5-(5S)-amino-5-carboxypentanoyl].

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