Involvement of Fis protein in replication of the Escherichia coli chromosome
- 1 January 1992
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 174 (2) , 398-407
- https://doi.org/10.1128/jb.174.2.398-407.1992
Abstract
We report evidence indicating that Fis protein plays a role in initiation of replication at oriC in vivo. At high temperatures, fis null mutants form filamentous cells, show aberrant nucleoid segregation, and are unable to form single colonies. DNA synthesis is inhibited in these fis mutant strains following upshift to 44 degrees C. The pattern of DNA synthesis inhibition upon temperature upshift and the requirement for RNA synthesis, but not protein synthesis, for resumed DNA synthesis upon downshift to 32 degrees C indicate that synthesis is affected in the initiation phase. fis mutations act synergistically with gyrB alleles known to affect initiation. oriC-dependent plasmids are poorly established and maintained in fis mutant strains. Finally, purified Fis protein interacts in vitro with sites in oriC. These interactions could be involved in mediating the effect of Fis on DNA synthesis in vivo.Keywords
This publication has 78 references indexed in Scilit:
- On the bacterial cell cycle: Escherichia coli mutants with altered ploidy.Genes & Development, 1990
- The Hin Invertasome: Protein-Mediated Joining of Distant Recombination Sites at the EnhancerScience, 1990
- Purification and DNA-binding properties of FIS and Cin, two proteins required for the bacteriophage P1 site-specific recombination system, cinJournal of Molecular Biology, 1987
- Fis binding to the recombinational enhancer of the Hin DNA inversion system.Genes & Development, 1987
- Hin-mediated site-specific recombination requires two 26 by recombination sites and a 60 by recombinational enhancerCell, 1985
- G inversion in bacteriophage Mu DNA is stimulated by a site within the invertase gene and a host factorCell, 1985
- On the process of cellular division in Escherichia coli: III. Thermosensitive Mutants of Escherichia coli Altered in the Process of DNA InitiationJournal of Molecular Biology, 1970
- Degree of superhelicity of covalently closed cyclic DNA's from Escherichia coliJournal of Molecular Biology, 1969
- The effect of amino acid deprivation on subsequent deoxyribonucleic acid replicationBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1963
- Thymine deficiency and the normal DNA replication cycle. IJournal of Molecular Biology, 1961