Hemolytic Action of Mouse Mammary Carcinoma Filtrate on Mouse Erythrocytes in vitro.

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Abstract
Filtrates (Seitz) prepared from 20% suspensions of mouse mammary carcinomas were mixed in test tubes with freshly prepared 1% oxalated suspensions of mouse erythrocytes in physiologic saline. After incubation at 37[degree]C for 21/2-3 hrs., hemolysis of the erythrocytes was observed. This hemolytic action of mouse mammary carcinoma filtrates was specific for the mouse erythrocytes, and did not occur when the filtrates were mixed with erythrocytes of rabbits, guinea pigs, or man. The hemolytic action of the tumor filtrates was not inhibited by the exposure of the filtrates to 56 [degree]C for 30 mins. prior to the test. Similar hemolytic action was observed when filtrates prepared from mouse lymphatic leukemia were mixed with mouse erythrocytes. Best results were observed when filtrates were prepared from spontaneous mammary carcinomas; mice of the C3H line were used. Transplanted tumors had a weaker hemolytic potency. For the leukemia tests, mice of the Ak line with either spontaneous or transplanted leukemia were used.