Penetration rate of glutaraldehyde in various buffers into plant tissue and gelatin gels

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Abstract
SUMMARY: The choice of buffer vehicle during fixation with glutaraldehyde influences not only the rate of crosslinking in a bovine serum albumin model system, but also the penetration rate of the glutaraldehyde into plant tissue and gelatin gels. The rate of penetration into plant tissue and the crosslinking rate of bovine serum albumin by glutaraldehyde are independent of each other in any one buffer. Buffer penetration into gelatin gels is not as fast as glutaraldehyde penetration into the same gels. The highest rate of crosslinking of bovine serum albumin by glutaraldehyde is obtained with a buffer vehicle consisting of Na2HPO4 and NaH2PO4. Glutarldehyde concentrations of 2–3% are advised for routine fixation of plant material.