Abstract
Fatty acid composition of phospholipid (PL) classes was measured in mouse testis. Among the long-chain polyenoic acids (LCPA), 22∶6 was found in highest concentration in phosphatidylethanolamine (PE), whereas percentages of 20∶4 and 22∶5 were not different in PE than in phosphatidylcholine. Each PL class had a unique fatty acid composition which was also different from that of triglycerides and cholesteryl esters. Differential metabolisms of 22∶5 and 22∶6 suggest different roles for these fatty acids in mouse testis. Tissue-specific functions of LCPA in mouse spermatogenesis may be divided between 22∶5 and 22∶6.

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