Kinetics of 2-Oxoglutarate Uptake by Synaptosomes from Bovine and Rat Retina and Cerebral Cortex and Regulation by Glutamate and Glutamine
- 1 January 1993
- journal article
- metabolic trafficking-in-energy-metabolism
- Published by S. Karger AG in Developmental Neuroscience
- Vol. 15 (3-5) , 330-335
- https://doi.org/10.1159/000111352
Abstract
2-Oxoglutarate (2-OG) is a metabolic precursor of glutamate and may be utilized to replenish the neurotransmitter pool. 2-OG is rapidly transported into neurons by a high-affinity carrier that is particularly prevalent in gluta-matergic terminals. Here we report the kinetics of [U-14C]2-OG uptake by crude synaptosomal preparations from bovine and rat retina and brain, and the modulatory effects of glutamate and glutamine. In all four tissues, 2-OG uptake was mediated by a high-affinity system (Kt ≈ 1 µM) that was subject to negative feedback control by glutamate and biphasic modulation by glutamine (another precursor of neurotransmitter glutamate).Keywords
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