Synthesis of dinucleoside tetraphosphates by RNA polymerase B (II) from calf thymus
- 2 December 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 193 (2) , 199-202
- https://doi.org/10.1016/0014-5793(85)80150-2
Abstract
Highly purified RNA polymerase B (II) from calf thymus catalyses the synthesis of dinucleoside tetraphosphates from ribonucleoside triphosphates in the absence of an oligonucleotide primer or additional protein factors. The reaction requires a DNA template and bivalent cations such as Mn2+ or Mg2+. It is strongly inhibited by heparin and high concentrations of α‐amanitin but not by rifampicin. On a given template various dinucleoside tetraphosphates of different sequence are formed although the yield depends on the nature of the template.Keywords
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