A coproofreading Zn2+-dependent exonuclease within a bacterial replicase
- 9 April 2006
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 13 (5) , 458-459
- https://doi.org/10.1038/nsmb1078
Abstract
The proofreading exonucleases of all DNA replicases contain acidic residues that chelate two Mg(2+) ions that participate in catalysis. DNA polymerase III holoenzymes contain their proofreading activity in a separate subunit, epsilon, which binds the polymerase subunit, alpha, through alpha's N-terminal php domain. Here we demonstrate that the alpha php domain contains a novel Zn(2+)-dependent 3' --> 5' exonuclease that preferentially removes mispaired nucleotides, providing the first example of a coediting nuclease.Keywords
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