Multiple column peptide synthesis, Part 2 (1, 2)
- 1 March 1993
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 41 (3) , 250-260
- https://doi.org/10.1111/j.1399-3011.1993.tb00333.x
Abstract
A manually operated apparatus for parallel multiple column soli-phase peptide synthesis is described. It employs Fmoc-amino acid-O-Dhbt or -Pfp esters in the continuous flow version of the polyamide method on small packed columns of kieselguhr supported resin in a reaction block of Teflon. The solvents and deprotecting reagents are dispensed from two washers in a parallel fashion and reagent consumption is low. Activated and protected amino acids are transferred from a dispenser tray as solutions, eight at a time. The use of the method is demonstrated by the synthesis of overlapping peptides from a protein structure and of analogous protease substrates. The products have been characterized by HPLC, FAB mass spectroscopy and amino acid analysis.Keywords
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