Fermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B
- 1 December 2005
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 27 (23-24) , 1891-1896
- https://doi.org/10.1007/s10529-005-3899-7
Abstract
Derivatives of ethanologenic Escherichia coli K011 were constructed for d(−)-lactate production by deleting genes encoding competing pathways followed by metabolic evolution, a growth-based selection for mutants with improved performance. Resulting strains, SZ132 and SZ186, contain native genes for sucrose utilization. No foreign genes are present in SZ186. Strain SZ132 also contains a chromosomally integrated endoglucanase gene (Erwinia chrysanthemi celY). Strain SZ132 produced over 1 mol lactate per liter of complex medium containing 10% (w/v) sugar (fermentation times of 48 h for glucose, 120 h for sucrose). Both strains produced 667–700 mmol lactate per liter of mineral salts medium. Yields for metabolized sugar ranged from 88% to 95% in both media.Keywords
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