Envelope Glycoproteins of HIV-1, HIV-2, and SIV Purified withGalanthus nivalisAgglutinin Induce Strong Immune Responses
- 1 May 1993
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 9 (5) , 431-438
- https://doi.org/10.1089/aid.1993.9.431
Abstract
Lectin affinity chromatography was used to purify in a single step the envelope glycoproteins of HIV-1, HIV-2, and SIV. Envelope glycoproteins carry the major determinants essential for protection by the humoral immune response. The purification of these proteins has previously been a laborious procedure. The glycoproteins were purified by a one-step procedure to a high level of purity by using Galanthus nivalis agglutinin (GNA). The purified glycoprotein had CD4-binding and antigenic reactivities. Strong immune responses to envelope proteins and peptides were seen in mice and primates after immunization with these preparations.Keywords
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