Identification of Frankia Strains by Two-Dimensional Polyacrylamide Gel Electrophoresis

Publisher Website
Google Scholar
Abstract
Fifteen Frankia strains from 5 different plant species were analyzed by 2-dimensional polyacrylamide gel electrophoresis to determine their relatedness by comparing the polypeptide patterns obtained. Thre major subgroups (A, C and D) were found in the Alnus-Comptonia-Myrica cross-inoculation group. An isolate from Purshia tridentata had a unique protein pattern and represents a distinct group of frankiae. Members of group A were isolated from root nodules of A. incana ssp. rugosa and A. viridis ssp. crispa. Group C organisms were from A. incana ssp. rugosa and C. peregrina nodules. Group D organisms were from A. incana ssp. rugosa, A. viridis ssp. cripsa and M. pensylvanica root nodules. Isolates from each gel group were obtained at several widely separated geographical locations. Two-dimensional polyacrylamide gel electrophoresis is apparently useful for identifying Frankia isolates.