Atomic force microscopy of oriented linear DNA molecules labeled with 5nm gold spheres
- 11 January 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 21 (1) , 99-103
- https://doi.org/10.1093/nar/21.1.99
Abstract
The atomic force microscope (AFM;1) can image DNA and RNA in air and under solutions at resolution comparable to that obtained by electron microscopy (EM) (2–7). We have developed a method for depositing and imaging linear DNA molecules to which 5nm gold spheres have been attached. The gold spheres facilitate orientation of the DNA molecules on the mica surface to which they are adsorbed and are potentially useful as internal height standards and as high resolution gene or sequence specific tags. We show that by modulating their adhesion to the mica surface, the gold spheres can be moved with some degree of control with the scanning tip.Keywords
This publication has 12 references indexed in Scilit:
- From Molecules to Cells: Imaging Soft Samples with the Atomic Force MicroscopeScience, 1992
- Imaging isolated strands of DNA molecules by atomic force microscopyUltramicroscopy, 1992
- Substrate preparation for reliable imaging of DNA molecules with the scanning force microscopeUltramicroscopy, 1992
- Atomic force microscopy for high-resolution imaging in cell biologyTrends in Cell Biology, 1992
- Atomic force microscopy of conventional and unconventional nucleic acid structuresJournal of Microscopy, 1992
- Atomic force microscopy of DNA moleculesFEBS Letters, 1992
- Circular DNA molecules imaged in air by scanning force microscopyBiochemistry, 1992
- Atomic force microscopy of single-and double-stranded DNANucleic Acids Research, 1992
- Imaging and nanodissection of individual supercoiled plasmids by atomic force microscopyNucleic Acids Research, 1992
- Atomic Force MicroscopePhysical Review Letters, 1986