Detection of Norwalk-like virus genes in the caecum contents of pigs
- 1 June 1998
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 143 (6) , 1215-1221
- https://doi.org/10.1007/s007050050369
Abstract
Norwalk-like virus genes were detected by RT-PCR in the caecum contents of pigs. Positive PCR products were produced from four out of 1 117 samples by nested PCR using human SRSV primers. Nucleotide sequences between 4 561 and 4 852 numbered according to the Norwalk virus genomic RNA in the RNA polymerase region were determined. Between the Norwalk virus sequence and the sequences detected in pigs, there was 58.2% to 59.9% sequence homology. The swine sequences were located on genogroup II of human SRSVs, but formed a subgroup in the phylogenetic tree of caliciviruses.Keywords
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