Binding and Elution of Serum Glycoproteins from Agarose
- 1 December 1984
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 14 (5) , 417-429
- https://doi.org/10.1080/00327488408061778
Abstract
Apparently, agarose is not an entirely inert support in affinity chromatography is presented. Eight percent granulated agarose adsorb serum glycoproteins in 10 mM phosphate buffer, pH 7.2 and desorb these in 200 mM NaCl in 10 mM phosphate buffer, pH 7.2. The desorbed glycoproteins retained their abilities to react with antibodies elicited in sheep to normal human serum glycoproteins, despite the loss of mannose and galactose following the agarose treatment.This publication has 2 references indexed in Scilit:
- Specific binding of bovine immunoglobulins to Sepharose and SephadexFEBS Letters, 1983
- Fractionation of protein mixtures on columns of granulated agarBiochimica et Biophysica Acta, 1961