Evaluation of Apple Juice Authenticity by Organic Acid Analysis
Open Access
- 1 November 1983
- journal article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 66 (6) , 1517-1520
- https://doi.org/10.1093/jaoac/66.6.1517
Abstract
An organic acid profile provides valuable information regarding authenticity of apple juice. The presence of D-malic acid is a clear indication of adulteration because this isomer does not occur naturally. Fumaric and citric acid levels above trace amounts are also inconsistent with pure apple juice; therefore, measurement of these organic acids may also be used as an authenticity check. Citric acid, total malic acid, and fumaric acid were determined in a single scan by liquid chromatography (LC) for 30 known pure apple juice samples. The L-isomer of malic acid was measured by an enzyme-specific method, and the D-isomer was calculated as the difference between total malic acid and L-malic acid.Keywords
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