Highly Specific Bacteriophage-associated Polysaccharide Hydrolases for Klebsiella aerogenes type 8

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Abstract
Two phage-bound polysaccharide hydrolases specific for K. aerogenes type 8 exopolysaccharides were isolated. Each enzyme was specific for the polysaccharide produced by the host strain. One enzyme hydrolyzed a pyruvylated and acetylated polymer, while the other only acted on the substrate lacking these substituents. Both enzymes were endogalactosidases releasing tetrasaccharides from their substrates which were only hydrolyzed to a limited extent.