Damage of DNA and its recovery in AH-109A cells treated with carboquone in vivo.

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Abstract
A single injection of carboquone at a dose of 0.1 mg/kg body weight induced damage of DNA of AH-109A rat hepatoma cells as revealed by alkaline and neutral sucrose density gradient centrifugation. A significant decrease in the sedimentation velocity of DNA on alkaline sucrose density gradient centrifugation was observed 15 min after injection, and it returned to that of control after 60 min. The size of DNA decreased progressively. When the cells were lysed with 2% sodium dodecyl sulfate solution and analyzed on neutral sucrose density gradient centrifugation, a similar change in the sedimentation velocity was observed. The results obtained from the studies on intraperitoneal growth of AH-109A cells pretreated with carboquone in vivo and survival of host animals corresponded to the extent of damage of DNA as revealed by alkaline and neutral sucrose density gradient centrifugation.

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