Effects of egg-yolk-citrate and egg-yolk-glycine dilutors on the morphology of bovine spermatozoa

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Abstract
Twenty semen samples were collected by means of the artificial vagina from three Friesian and two Dexter bulls. In each sample, thehead lengthandhead breadthof spermatozoa which did not take up the nigrosin-eosin stain, and their percentage before and after 72 hr. of preservation in egg-yolk-citrate and egg-yolk-glycine, were studied. The following results were obtained from the study:1. No significant variation was found in the two measurements of sperm head before and after preservation in E.Y.C.2. Both thehead lengthandhead breadthof spermatozoa preserved in E.Y.G. were significantly less than those before and after preservation in E.Y.C..3. The change in size of the spermatozoa head in E.Y.G.. dilutor may be due to an alteration in the acrosome.