Binding of Calcium to Serum Albumin III. Influence of Ionic Strength and Ionic Medium
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 29 (4) , 427-432
- https://doi.org/10.3109/00365517209080262
Abstract
A marked inverse relation between calcium binding to serum albumin and ionic strength has been demonstrated by means of an ultrafiltration method and shown to be identical in NaCl and KCl medium, while magnesium binds competitively to albumin with the same affinity as calcium ions. The ionic strength dependence has been resolved into a linear relation between log calcium ion activity coefficient and log apparent intrinsic association constant, whose extrapolated value at zero ionic strength of supporting electrolyte is 1445 ± 67 litres/mole. The importance of constant ionic strength in binding studies is emphasized.Keywords
This publication has 22 references indexed in Scilit:
- Vitamin D3-induced Calcium-binding ProteinJournal of Biological Chemistry, 1971
- The Thermodynamic Activity of Calcium Ion in Sodium Chloride-Calcium Chloride ElectrolytesBiophysical Journal, 1968
- Partition of calcium, phosphate, and protein in the fluid phase aspirated at calcifying sites in epiphyseal cartilageJournal of Clinical Investigation, 1968
- APPLICATIONS OF MEMBRANE ELECTRODES*Annals of the New York Academy of Sciences, 1968
- Binding of calcium by human plasma proteins under simulated physiologic conditionsJournal of Applied Physiology, 1964
- The Interactions of Small Ions with Proteins from Electrical Conductance and'Transference Experiments.The Journal of Physical Chemistry, 1958
- Some General Aspects of Molecular InteractionsPublished by Elsevier ,1958
- Competitive Binding of Calcium and Magnesium with Serum Albumin.Experimental Biology and Medicine, 1955
- Studies on the binding of small ions in protein solutions with the use of membrane electrodes. II. The binding of calcium ions in solutions of bovine serum albuminArchives of Biochemistry and Biophysics, 1953
- Individual Activity Coefficients of Ions in Aqueous SolutionsJournal of the American Chemical Society, 1937