Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa Nodulation
Open Access
- 1 August 1999
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 12 (8) , 748-751
- https://doi.org/10.1094/mpmi.1999.12.8.748
Abstract
A gene encoding chitinase from Serratia marcescens BJL200 was cloned into a broad-host-range vector (pRK415) and mobilized into Sinorhizobium fredii USDA191. Chitinolytic activity was detected in S. fredii USDA191 transconjugants that carried the S. marcescens chiB gene. Chitinase-producing S. fredii USDA191 formed nodules on soybean cultivar McCall. However, there was a delay in nodule formation and a marked decrease in the total number of nodules formed by the chitinase-producing S. fredii in comparison with the wild-type strain. Expression of chitinase in S. meliloti RCR2011 also impeded alfalfa nodulation. Thin-layer chromatography of 14C-labeled Nod factors from chitinase-producing S. fredii USDA191 revealed hydrolysis of lipochitooligosaccharides.Keywords
This publication has 18 references indexed in Scilit:
- Rhizobium fredii synthesizes an array of lipooligosaccharides, including a novel compound with glucose inserted into the backbone of the moleculeFEBS Letters, 1996
- Structurally diverse chitolipooligosaccharide Nod factors accumulate primarily in membranes of wild-type Rhizobium leguminosarum biovar trifoliiBiochemistry, 1995
- Structures of Nodulation Factors from the Nitrogen-Fixing Soybean Symbiont Rhizobium fredii USDA257Biochemistry, 1994
- Expression ofSerratia marcescensChitinase Gene inRhizobium melilotiDuring Symbiosis on Alfalfa RootsMolecular Plant-Microbe Interactions®, 1993
- Transgenic Plants with Enhanced Resistance to the Fungal Pathogen Rhizoctonia solaniScience, 1991
- Control of Plant Diseases by Chitinase Expressed from Cloned DNA inEscherichia coliPhytopathology®, 1989
- Plant chitinases are potent inhibitors of fungal growthNature, 1986
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Evaluation of effectiveness in mutant strains of rhizobium by acetylene reduction relative to other criteria of N2 fixationPlant and Soil, 1970
- The chitinase of Serratia marcescensCanadian Journal of Microbiology, 1969