PHYSICAL CONSTANTS AND ESTIMATION OF STEREOISOMERIC 2,3-BUTANEDIOLS

Abstract
The refractive index – temperature relation for optically active and racemic diols is linear, with from 25° to 40°C.: for the meso-isomer from 25° to 50°C. The specific rotation . Measurements of and in suitably purified mixtures of stereoisomers allow calculation of the percentage of each isomer present. Tests of the method on known mixtures of all three stereoisomers show an experimental error of ±3%. Analyses of five diol samples from pilot plant fermentations of beet molasses by Aerobacter aerogenes reveal 65–87% meso-, from 2 to 16% dextro-, and from 0 to 33% racemic 2,3-butanediol.

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