Starch Gel Electrophoresis of Plant NADH-Nitrate Reductase and Nitrite Reductase

Abstract
Isozymes of both nitrate reductase (NR) and nitrite reductase (NiR) have been found in plant tissues, mainly after partial purification. We have used starch gel electrophoresis to examine both NR and NiR in crude extracts. Only one NR and one NiR enzyme were found in wheat tissues and no difference in mobilities could be detected between root and leaf enzymes. It was confirmed that some tissues of corn have two NiR isozymes.

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