Abstract
Growth of tobacco seedlings in aseptic cultures under constant light and temp. was greatly modified by isoleucine in the culture medium. It caused loss of dominance of the apical bud, numerous narrow leaves and mottled chlorosis. These signs resemble those of a tobacco disease called frenching. The severity of symptoms varied with the conc. of isoleucine. No other amino acid, sugar, vitamin or peptone tested caused the reaction.

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