A STUDY OF ADDED GM-CSF INDEPENDENT GRANULOCYTE AND MACROPHAGE PRECURSORS IN MOUSE SPLEEN INFECTED WITH MYELOPROLIFERATIVE SARCOMA-VIRUS (MPSV)

  • 1 January 1982
    • journal article
    • research article
    • Vol. 10  (4) , 373-382
Abstract
A subpopulation of granulocyte and macrophage precursors (GM-CFUc) differentiating in the agar colony technique of Bradley and Metcalf into mature granulocytes and macrophages, without the addition of granulocyte and macrophage colony stimulating factor (GM-CSF), was detected in MPSV [myeloproliferative sarcoma virus] infected mice. These precursors were detected 5 days after virus infection, reaching a maximal concentration of 1/10,000 spleen or bone marrow cells, 25 days after viral infection. The number of the added GM-CSF independent GM-CFUc was linearly correlated with the number of seeded MPSV hematopoietic cells. No GM-CSF producing cells could be detected in the MPSV spleen using normal bone marrow GM-CFUc as responder cells. Study of the GM-CSF sensitivity of the GM precursors demonstrated the existence of 2 GM-CFUc populations in the MPSV spleen as follows: a GM-CSF dependent population with a GM-CSF sensitivity similar to that of normal GM-CFUc and a GM-CFUc population which differentiated in the absence of detectable amount of GM-CSF and of which differentiation was not affected by the addition of progressive amounts of GM-CSF. A possible model explaining these results is proposed.

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