Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg
- 31 January 1988
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (2) , 653-656
- https://doi.org/10.1128/jb.170.2.653-656.1988
Abstract
A wild-type strain of Methanobacterium thermoautotrophicum Marburg was transformed by DNA from strains resistant to 5-fluorouracil. Recipient cells were grown without selection on gellan gum (GELRITE) plates with DNA. Drug-resistant cells were recovered by replica plating the resulting colonies onto drug plates. Transformation required high-molecular-weight DNA with appropriate markers and was not observed on agar or in liquid media under a variety of conditions.This publication has 30 references indexed in Scilit:
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