Effect of Storage Temperature on the Activity of Superoxide Dismutase, Catalase, Glutathione Peroxidase, Glutathione Reductase and Glutathione S-Transferase in Rat Liver and Kidney Homogenates
- 1 July 1993
- journal article
- research article
- Published by S. Karger AG in Enzyme and Protein
- Vol. 47 (3) , 149-155
- https://doi.org/10.1159/000468670
Abstract
The behavior of the catalytic activities of the enzymes superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and glutathione reductase was tested in rat liver and kidney homogenates stored at 4, -20 and -70°C and in corresponding tissue samples stored at -70°C. The stabilities of enzymes were different for various enzymes and were dependent on the organ (liver, kidney) and the storage temperature. The storage temperature of -70°C guaranteed the best stability and the five enzymes investigated were sufficiently stable in preserved tissue samples or in homogenates prepared with conventional mannit/sucrose homogenization solution. Under such conditions, these enzymes were stable over at least 4 or 1 weeks, respectively.Keywords
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