Abstract
The response to red and far-red light of intact and punched seeds of Lactuca sativa var. Grand Rapids is compared. Punched seeds are fully responsive to red light after only 1 min of hydration; intact seeds require 15 min. Punching has no effect on the usual red and far-red light mediation of germination of these seeds. It does, however, raise the level of dark germination, indicating that there are at least two mechanisms by which the seed can germinate, only one of which is controlled by phytochrome.Treatment of seeds with dichloromethane enhances germination in seeds imbibed 1 min but not the penetration of arsenate, which is normally excluded by the intact seed. The detergent Brij greatly enhances the penetration of arsenate into the seed.

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