Primary cultures of dispersed hypothalamic cells from fetal rats: Morphology, electrical activity, and peptide content
- 1 July 1980
- journal article
- research article
- Published by Wiley in Journal of Neurobiology
- Vol. 11 (4) , 417-424
- https://doi.org/10.1002/neu.480110408
Abstract
Cultures prepared from dispersed fetal hypothalamic tissue have cells which can be identified as neurons by their morphology and electrical activity. The elongation of neuritic processes in these cultures is increased by treatment with 1‐β‐D‐arabinofuranosylcytosine (ara‐C). Hypothalamic cultures have measurable quantities of immunoreactive substance P and neurotensin, and the neurons can accumulate (3H)GABA.This publication has 15 references indexed in Scilit:
- Rat cortical neurons in cell culture: Culture methods, cell morphology, electrophysiology, and synapse formationBrain Research, 1978
- The maintenance and identification of mouse cerebellar granule cells in monolayer cultureBrain Research, 1977
- THE AROMATIZATION OF ANDROGENS BY PRIMARY MONOLAYER CULTURES OF FETAL RAT HYPOTHALAMUSEndocrinology, 1977
- Identification of catecholamine and luteinizing hormone-releasing hormone (LHRH)-containing neurons in primary cultures of dispersed cells of the basal hypothalamusBrain Research, 1977
- Dissociated cell cultures from fetal mouse hypothalamus patterns of organization and ultrastructural featuresExperimental Brain Research, 1975
- Brain Cells in Culture: Morphological Transformation by a ProteinScience, 1974
- Electrophysiologic and morphologic properties of neurons in dissociated chick spinal cord cell culturesDevelopmental Biology, 1974
- Synapse formation between dissociated nerve and muscle cells in low density cell culturesDevelopmental Biology, 1972
- Screening for neurotransmitters: A rapid radiochemical procedureJournal of Neurobiology, 1971
- Stimulation of Salivary Secretion by a Factor Extracted from Hypothalamic Tissue1Endocrinology, 1967