Effect of ascorbic acid on the synaptosomal uptake of [3H]MPP+, [3H]Dopamine, and [14C]GABA
- 1 January 1987
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 17 (3) , 298-301
- https://doi.org/10.1002/jnr.490170315
Abstract
The effects of ascorbic acid on the synaptosomal uptake of [3H]MPP+, [3H]dopamine, and [14C]GABA were examined in attempts to understand the mechanism of ascorbic acid attenuation of MPTP neurotoxicity. [3H]Dopamine uptake was increased at lower levels (0.01 and 0.1 mM) and decreased at higher levels (10 mM) of ascorbic acid. Ascorbic acid inhibited [3H]MPP+ uptake (IC50 = 0.1 mM) and [14C]GABA uptake (IC50 = 10 mM). Washout of ascorbic acid restored uptake of [3H]dopamine and [3H]MPP+, suggesting that ascorbic‐acid‐induced lipid peroxidation was not involved in the effect on uptake. In addition to the possible involvement of antioxidant mechanisms in the in vivo attenuation of the neurotoxicity of MPTP by ascorbic acid, the present results indicate a direct effect of ascorbic acid in inhibiting the uptake of MPP+ into dopaminergic nerve terminals.Keywords
This publication has 13 references indexed in Scilit:
- Protection against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine neurotoxicity by the antioxidant ascorbic acidNeuropharmacology, 1985
- Parkinsonism-inducing neurotoxin, N-methyl-4-phenyl-1,2,3,6 -tetrahydropyridine: uptake of the metabolite N-methyl-4-phenylpyridine by dopamine neurons explains selective toxicity.Proceedings of the National Academy of Sciences, 1985
- Uptake of MPP(+) by dopamine neurons explains selectivity of parkinsonism-inducing neurotoxin, MPTPEuropean Journal of Pharmacology, 1984
- Rapid determination of dopamine uptake in synaptosomal preparationsJournal of Neuroscience Methods, 1983
- Ascorbic acid could be hazardous to your experiments: A commentary on dopamine receptor binding studies with speculation on a role for ascorbic acid in neuronal functionNeuropharmacology, 1983
- Dopamine Uptake by Rat Striatal Synaptosomes: Time‐and Temperature‐Dependent Decay and Protection by Dithiothreitol and DopamineJournal of Neurochemistry, 1983
- Simultaneous electrochemical and unit recording measurements: Characterization of the effects of d-amphetamine and ascorbic acid on neostriatal neuronsBrain Research, 1983
- Dopamine uptake in striatal synaptosomes exposed to peroxidation “in vitro”Biochemical Pharmacology, 1983
- Inhibitory Effects of Ascorbic Acid on the Binding of [3H]Dopamine Antagonists to Neostriatal Membrane Preparations : Relationship to Lipid PeroxidationJournal of Neurochemistry, 1982
- INHIBITION PATTERN BY ANALOGS INDICATES THE PRESENCE OF TEN OR MORE TRANSPORT SYSTEMS FOR AMINO ACIDS IN BRAIN CELLSJournal of Neurochemistry, 1979