Chlorophyll interference with phytochrome measurement
- 1 January 1977
- journal article
- conference paper
- Published by Springer Nature in Planta
- Vol. 135 (2) , 119-123
- https://doi.org/10.1007/bf00387159
Abstract
Measurements of phytochrome by Δ (ΔA725–815 nm) were completely suppressed at chlorophyll concentrations of the order of 20–40 μg g-1 f.wt. in vivo and 37 μg cm-3 in vitro, and the readings were reduced by 50% at only 12 μg cm-3 in vitro. At these concentrations of chlorophyll in aqueous methanol, the loss of phytochrome signal in vitro appeared to be due to failure of phytochrome photoconversion rather than to interference with ΔA measuremebt by chlorophyll fluorescence in the 125/815 nm measuring beam.This publication has 7 references indexed in Scilit:
- Influence of chlorophyll content on phytochrome measurements in turnip cotyledonsPlanta, 1977
- Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form.Proceedings of the National Academy of Sciences, 1975
- An immunochemical characterization of the phytochrome destruction reactionBiochimica et Biophysica Acta (BBA) - Protein Structure, 1974
- The influence of chlorophyll on in-vivo difference spectra of phytochromePlanta, 1972
- Increased phenylalanine ammonia-lyase activity due to light treatment and its significance for the mode of action of phytochromePhytochemistry, 1970
- A GENERAL HYPOTHESIS TO INTERPRET ‘HIGH ENERGY PHENOMENA’ OF PHOTOMORPHOGENESIS ON THE BASIS OF PHYTOCHROMEPhotochemistry and Photobiology, 1966
- Properties of PhytochromeAnnual Review of Plant Physiology, 1965