Abnormal proteins of shortened length are preferentially degraded in the cytosol of cultured MRC5 fibroblasts
- 12 March 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 168 (1) , 134-138
- https://doi.org/10.1016/0014-5793(84)80222-7
Abstract
Puromycyl peptides were degraded in MRC5 fibroblasts more rapidly than normal proteins labelled for the corresponding length of time for both long and short labelling periods. The degradation of the puromycyl peptides occurred almost exclusively in the cytosol of the cells. Even when the half‐lives of normal and puromycyl peptides were manipulated to be similar, proportionally more of the normal proteins were degraded in the lysosomes. The rapid degradation of the puromycyl peptides was not due to the inhibition of protein synthesis brought about by puromycin but was due to the structure of the substrates themselves. The degree and intracellular site of degradation of puromycyl peptides closely mimic those of abnormal (missense) proteins containing amino acid analogues.Keywords
This publication has 14 references indexed in Scilit:
- Subcellular distribution of abnormal proteins in rabbit reticulocytesFEBS Letters, 1983
- Effects of ATP and cell development on the metabolism of high molecular weight aggregates of abnormal proteins in rabbit reticulocytes and cell-free extractsBiochimica et Biophysica Acta (BBA) - General Subjects, 1982
- Degradation of abnormal proteins in growing and in quiescent fibroblasts in cultureFEBS Letters, 1981
- Proteolysis of puromycin-peptides in rabbit reticulocytes: detection of a high molecular weight oligopeptide proteolytic substrateFEBS Letters, 1980
- The effect of protease inhibitors and decreased temperature on the degradation of different classes of proteins in cultured hepatocytesJournal of Cellular Physiology, 1979
- Selective control of the degradation of normal and aberrant proteins in Reuber H35 hepatoma cellsBiochemical Journal, 1976
- Degradation of abnormal proteins in heLa cellsJournal of Cellular Physiology, 1976
- Degradation of Abnormal Proteins in Escherichia coli: Relative Susceptibility of Canavanyl Proteins and Puromycin Peptides to Proteolysis in vitroEuropean Journal of Biochemistry, 1974
- Rapid degradation of puromycyl peptides in hepatoma cells and reticulocytesFEBS Letters, 1974
- Degradation of Abnormal Proteins in Escherichia coliProceedings of the National Academy of Sciences, 1972