Characterization of monoclonal antibodies against avian reovirus strain S1133
- 1 March 1996
- journal article
- Published by Taylor & Francis in Avian Pathology
- Vol. 25 (1) , 57-67
- https://doi.org/10.1080/03079459608419120
Abstract
Monoclonal antibodies (MAbs) were produced against a vaccinal S1133 strain of avian reovirus. Characterization of six MAbs in Western blotting, radioimmunoprecipitation and gold immunoelectron microscopy revealed that the MAbs were specific to the outer capsid proteins, mu2/mu2c, sigma2 and sigma3. Two of three MAbs, directed against sigma2 protein, neutralized the virus infectivity in a broadly specific manner, whereas the third had no neutralizing activity. The only MAb which reacted with sigma3 protein showed a type-specific neutralizing activity. Two MAbs recognizing the mu2/mu2c proteins failed to neutralize the virus infectivity.Keywords
This publication has 14 references indexed in Scilit:
- Cloning, sequencing and expression of the S1 gene of avian reovirusJournal of General Virology, 1995
- Type‐specific antigenicity of avian reovirusesAvian Pathology, 1995
- Interaction of avian reovirus with chicken lymphoblastoid cell linesAvian Pathology, 1994
- Identification of Proteins Encoded by Avian Reoviruses and Evidence for Post-translational ModificationVirology, 1993
- The Application of Immunogold Silver Staining (IGSS) for the Detection of Transmissible Gastroenteritis Virus in Fixed TissuesJournal of Veterinary Diagnostic Investigation, 1993
- Classification and Nomenclature of VirusesPublished by Springer Nature ,1991
- Characteristics and analysis of electropherotypes of avian reovirus field isolatesVeterinary Microbiology, 1990
- Protein coding assignment of the S genes of the avian reovirus S1133Virology, 1985
- The interaction of a series of hybridoma IgGs with reovirus particles Demonstration that the core protein λ2 is exposed on the particle surfaceVirology, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970