Development of Amsacta moorei Entomopoxvirus in Ovarian and Hemocyte Cultures from Estigmene acrea Larvae

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Abstract
Primary ovarian and hemocyte tissue cultures prepared from Estigmene acrea larvae were infected with Amsacta moorei entomopoxvirus. Infection of cultures was achieved with either purified virus or virus-containing culture medium from infected cultures. Virus replication and formation of virus-containing inclusions (VCI) were similar to that normally observed in vivo except that fewer virions appeared to be occluded in VCI.

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